Nume proiect

Nume proiect: Substraturi hibride biodegradabile si antibacteriene cu morfologie controlata si bio-activare localizata

Cod proiect: TE_43

Denumirea Programului din PN II: Resurse Umane

Subprogram: Proiecte de cercetare pentru stimularea constituirii de tinere echipe de cercetare independente

Autoritatea contractanta: Unitatea Executiva pentru Finantarea Invatamantului Superior, a Cercetarii, Dezvoltarii si Inovarii (UEFISCDI)

Director de proiect: Valentina Dinca

Coordonator: Institutul National pentru Fizica Laserilor, Plasmei si Radiatiei

Obiectivele fazelor de executie:

v Adaptarea sistemelor MAPLE, LIFT si iradiere a materialului pentru obtinerea de platforme hibride biodegradabile de studiu celular in vitro.

v Obtinerea de filme subtiri polimerice biocompatibile, antimicrobiene si degradabile prin MAPLE si spin coating (WP2);

v Obtinerea de microstructuri polimerice cu topografii controlate (santuri/canale, cratere) prin iradierea cu laseri (WP3);

v Obtinerea de microstructuri polimerice specific localizate controlate prin LIFT (matrici de pixeli polimerici) (WP3);

v Transferul localizat de factori bioactivi prin LIFT (WP 4);

v Testarea filmelor subtiri si a microstructurilor obtinute in vitro (WP5)

v Diseminare si raportare faza proiect (WP6).

Diseminare proiect

Prezentari orale:

MAPLE and LIFT “soft” laser techniques for organic materials processing, V. Dinca, A. Matei, A. Palla Papavlu and M. Dinescu , la workshop-ul Curent trends and advanced ellipsometry and all x-ray techniques for the characterization of TCO, BIO and other nanostructured materials”, Lectie invitata, 12-14 Septembrie, 2012, Bucuresti, Romania
Laser induced forward transfer (LIFT) for biomedical applications, V. Dinca, A. Palla Papavlu, M. Dinescu, „Emerging Analytical Tools to Investigate Nitro-Oxidative Stress” - Exploratory Workshop, Iulie 24-26 2012,Bucuresti, Romania- ‘lectie invitata
Biointerfaces engineering using laser based methods for biomedical applications-HPLA/BEP Santa Fe, 18-29 aprilie 2014, lectie invitata
Designed surfaces topography for influencing mesenchymal stem cells differentiation in the absence of active stimulation factors, V Dinca, Seminar, EMPA, THUN, Switzerland, 14 iunie 2014

Postere prezentate:

1. V. Dinca, L. Rusen, M. Dinescu, Matrix Assisted Pulsed Laser Evaporation of biopolymers using 266 nm for biomedical applications, ROMOPTO 3-6 sept 2012, Bucuresti,Romania

2. L. Rusen, V. Dinca, C. Mustaciosu, M. Dinescu, Effective method for polymeric materials patterning for cell behavior studies, ROMOPTO 3-6 sept 2012, Bucuresti,Romania

3. L. Rusen, V. Dinca , C. Luculescu, M. Filipescu, M. Zamfirescu and M. Dinescu, Morphological characteristics of chitosan based structures obtained by femtosecond laser methods, EMRS 2012, Strasbourg, Franta

4. V. Dinca, C. Mustaciosu, A. Palla-Papavlu, L. Rusen, B. Mitu, M. Filipescu , M. Dinescu, Protein-Resistant Polymer Coatings obtained by Matrix Assisted Pulsed Laser Evaporation, EMRS 2012, Strasbourg, Franta

5. Dinca V., Rusen L., A. Palla Papavlu, A. Matei, V. Ion and M. Dinescu, Polymer Multilayer obtained by Matrix Assisted Pulsed Laser Alternative Evaporation, EMRS 2012, Strasbourg, Franta

6. V. Dinca, T. Mattle A. Palla Papavlu, M. Filipescu, M. Dinescu, , A. Wokaun, T. Lippert, Polymer micro and nanopatterning using laser transfer onto soft substrates assisted by a dynamic release layer, EMRS 2012, Strasbourg, Franta

7. Laurentiu Rusen, Marian Zamfirescu, Catalin Luculescu and Valentina Dinca, International conference of Student Chapter Photoniscs, ISCP, mai, 2012, Sinaia, Morphological Changes on the Surface of Polymer Films Induced by Femtosecond
Laser Irradiation

8. V. Dinca, A.Palla-Papavlu, M. Filipescu, L. Rusen, T.Lippert, M. Dinescu, LIFT and MAPLE Methods for Obtaining Multicomponent Protein-Polymer Patterns with Controlled Cell Binding Properties, 12th International Conference on Laser Ablation, October, 6th-11th, 2013, Ischia (Italy)- poster presentation P3-61 COLA 2013

9. P. Florian, V. Dinca, L. Sima, L. Rusen, M. Dinescu, R. Evans, A. Roseanu, Lactoferin-Cispaltin-Embedded Biodegradable Polymeric Thin Films With Antitumoral Activity in Vitro, International BioIron Society Program, University College London, April 14 - 18, UK - poster presentation BioIron2013-P52

10. V. Dinca, L. Rusen, C. Mustaciosu and M. Dinescu, Protein Absorption and Cell Attachment on copolymer thin films obtained by MAPLE Modern Laser Applications - INDLAS 2013, Bran, 20 - 24 May, Romania - poster presentation INDLAS2013

11. L. Rusen, V. Dinca, C. Mustaciosu, M. Zamfirescu, and M. Dinescu, The study of the influence of biopolymer patterns on cell behavior in vitro, Modern Laser Applications - INDLAS 2013, Bran, 20 - 24 May, Romania - poster presentation INDLAS2013

12. V. Dinca, L. Rusen, A. Palla-Papavlu, C. Mustaciosu, B. Mitu, M. Filipescu and M. Dinescu, Matrix assisted pulsed laser evaporation (MAPLE) of bioactive factors for stimulated cell adhesion interfaces, European Materials Research Society EMRS2013, 27-31 May, Strasbourg - poster presentation VPI31_EMRS2013

13. L. Rusen, M. Filipescu, V. Dinca and M. Dinescu,Stimuli responsive functional polymeric thin films obtained by matrix assisted pulsed laser evaporation (MAPLE) for cell detachment studies European Materials Research Society EMRS2013, 27-31 May, Strasbourg - poster presentation Best Poster Award Symposium V VPI33_EMRS2013

14. L. Rusen, V. Dinca, C. Luculescu, M. Filipescu, C. Mustaciosu, M. Bacalum, M. Zamfirescu, M. Dinescu , Tailored topography control of biopolymer surfaces by ultrafast lasers for cell - substrate studies, European Materials Research Society EMRS2013, 27-31 May, Strasbourg - poster presentation VPII28_EMRS2013

15. L. Rusen, V. Dinca , A. Palla Papavlu, C. Luculescu, M. Filipescu, L. Sima, A. Roseanu, T. Lippert and M. Dinescu, Micro-/nano-engineering surfaces using laser based methods for directing cell behavior at the cell–biomaterial interface in vitro, ISCP, 2014, Orastie, Romania

16. M. Icrivezi, J. Banita, V. Dinca, E Siringil, K. Bohlen, K.Wasmer, M. Dinescu, P. Hoffmann, A. Roseanu , Inflammatory response of laser micro- structured surfaces, ICPAM-10, 21-28 septembrie, Iasi, Romania

17. V. Dinca, P.Alloncle, P.Delaporte, L. Rusen, C. Mustaciosu, V. Ion, M. Bacalum, M. Dinescu, Antimicrobial and degradable biohybrid substrates with controlled surface architecture, ICPAM-10, 21-28 septembrie, Iasi, Romania

18. V. Dinca, L. Sima, E. Siringil, K. Bohlen, K. Wasmer, M. Dinescu, P. Hoffmann Topographical cues for modulating Mesenchymal stem cells fate, , ICPAM-10, 21-28 septembrie, Iasi, Romania

19. L.E. Sima, V. Dinca*, L. Rusen, M. Cazan, M. Chiritoiu, A. Palla-Papavlu, M. Dinescu, Combined micro- and nanoscale topography on Zirconia surfaces modulates human mesenchymal stem cells growth and cell nuclei alignment, 26-30 mai , EMRS, Lille, Franta, Symposium JP.XI 19, EMRS, Lille, Franta, 2014

20. V. Dinca, P. Florian, M. Icrivezi, L. Rusen, A. Palla-Papavlu, A. Roseanu and M. Dinescu, Hybrid and biodegradable polymeric thin films effect on murine melanoma B16-F10 cells, 26-30 mai , EMRS, Lille, Franta, Symposium NPII 11_EMRS, 2014

Articole publicate:

1. L.Rusen, M.Cazan, C.Mustaciosu, M.Filipescu, S.Sandel, M.Zamfirescu, V.Dinca, M.Dinescu, Tailored topography control of biopolymer surfaces by ultrafast lasers for cell - substrate studies, Applied Surface Science, Volume 302, 30 May 2014, Pages
256-261

2. L.Rusen, V.Dinca, B.Mitu, C.Mustaciosu, M.Dinescu, Temperature responsive functional polymeric thin films obtained by Matrix Assisted Pulsed Laser Evaporation for cells attachment-detachment study, Applied Surface Science, Volume 302, 30 May
2014, Pages 134-140

3. V.Dinca, P.E.Florian, L.E.Sima, L.Rusen, C.Constantinescu, R.W.Evans, M.Dinescu, A.Roseanu, MAPLE-based method to obtain biodegradable hybrid polymeric thin films with embedded antitumoral agents, Biomedical Microdevices; February 2014, Volume 16, Issue 1, pp 11-21

4. L.Rusen, C.Mustaciosu, B.Mitu, M.Filipescu, M.Dinescu, V.Dinca, Protein-Resistant Polymer Coatings obtained by Matrix Assisted Pulsed Laser Evaporation, Applied Surface Science DOI: 10.1016/j.apsusc.2013.01.109, Volume 278, 1 August 2013, Pages 198-202.

5. V.Dinca, T.Mattle, A.Palla-Papavlu, L.Rusen, C.Luculescu, T.Lippert, M.Dinescu, Polyethyleneimine patterns obtained by laser-transfer assisted by a Dynamic Release Layer onto Themanox soft substrates for cell adhesion study, Applied Surface Science DOI: 10.1016/j.apsusc.2013.02.052, Volume 278, 1 August 2013, Pages 190-197.

6. M. Cazan, V. Dinca, M. Dinescu, C. R. Popescu, Zirconia, a wonderful material, practica Medicala – VOL. IX, NR. 2(34), AN 2014

7. Laser processing of organic materials: applications in tissue engineering and chemical sensing, M. Dinescu1, A. Matei, V. Dinca, A. Palla Papavlu, F. Di Pietrantonio, D. Cannata, M. Benetti, E. Verona, T. Lippert, Romanian Reports in Physics, Vol. 65, No. 3, P. 1019–1031, 2013

Etapa I - 15.12.2011 (click pentru raportul stiintific al etapei)

A.1.1 Adaptarea sistemelor MAPLE, LIFT si ablatie directa a materialului pentru obtinerea de platforme hibride biodegradabile de studiu celular in vitro.

A.1.2 Analiza bibliografica. Pagina web. Achizitionarea de materiale si echipamente.

Valoare etapa I: 114,375 lei

Etapa II - 5.12.2012 (click pentru raportul stiintific al etapei)

Valoare etapa II: 220,000 lei

Etapa III - 5.12.2013 (click pentru raportul stiintific al etapei)

Etapa IV - 30.09.2014 (click pentru raportul stiintific al etapei)

Valoare etapa IV: 178,750 lei