Project name: “Multiscale bone-like intelligent interfaces engineering using laser methods for mesenchymal stem cells enhanced osteoinduction”

Acronym: BIOSINTEL
Contract No.: 24/2015
Project code: PN-II-RU-TE-2014-4-2434 
Contracting authority: Executive Agency for Financing Higher Education , Research, Development and Innovation ( UEFISCDI )
Financing source: State budget
Project team:
    Dinca Valentina - project director
    Rusen Laurentiu
    Filipescu Mihaela
    Palla Papavlu Alexandra
    Brajnicov Simona
    Sima Livia Elena
    Madalina Icriverzi
    Scarisoreanu Nicu Doinel
    Dinescu Maria
 
Project Summary: BIosINTEL project aim is engineering smart bio-interfaces with multiple and synergetic functionalities for effective controlled regulation of stem cell differentiation and low inflammatory response for enhanced osseointegration. The innovative strategy within the project proposed is:
-Smart surface design: multiscaled microstructured surfaces obtained by laser processing technique
-Innovative hierarchical nanostructuring of the microstructured surfaces by tailoring the self assembling of biocompounds and biodegradable polymers spherulites formation
-Specific laser based functionalization of surfaces (by matrix assisted pulsed laser evaporation and LIFT)
-Effective use of hierarchically patterned topography for tuning biointerface characteristics to stem cell differentiation.
The design model based on tailored topographical and chemical cues for quantifying the steering of stem cell has impact in basic cell researches and tissue engineering application.
 

General objective:

The aim of BIosINTEL is the engineering of smart bio-interfaces with multiple and synergetic functionalities for effective controlled regulation of stem cell differentiation and low inflammatory response for enhanced osseointegration.


Stage 1: october 2015-december 2015

Value: 90.000 lei

Objectives:

1. Smart surface design: Bone like multiscaled microstructured surfaces(pits with various parameters and density on the surface) obtained by masks assisted laser processing.

2. Web page.

Dissemination:

Invited lecture: "Multiscaled Bone-Like Intelligent Interfaces Engineering using Laser Methods for Steering Mesenchymal Stem Cells Behavior in vitro" HPLA-DE 4-7 April 2016, Santa Fe, NM, USA


Stage 2
Value: 209.630,00 lei

Objectives
Ob. 2.1 Microstructured surfaces obtained by laser ablation assisted by masks
Ob. 2.2 Hierarchicaly micro and nanostructured surfaces: obtaining and analyses
Ob.2.3 Preliminary evaluation of macrophages and stem cells with the microstructured materials

Dissemination
Invited presentations:
• V. Dinca, L. E. Sima, M. Icriverzi, L. Rusen, A. Bonciu, K. Wasmer, E. C. Siringil, A.Roseanu, A.Campean, P. Hoffmann and M. Dinescu, Multiscale bone-like intelligent interfaces engineering using laser methods for steering mesenchymal stem cells behaviour in vitro, HPLA DE 2016, Promising New Laser and Optical Technologies Symposium, April 4-7, Santa Fe, USA
• V. Dinca, L. E. Sima, L. Rusen, M. Icriverzi, A. Bonciu, I.Anghel, A. Roseanu, P.Hoffmann, M.Dinescu, Materials and laser technologies for controlled bio-interfaces in tissue engineering applications -7-14 september-ICPAM 2016, Cluj, Romania
• L. Rusen, M. Icriverzi, C. Mustaciosu, V. Dinca, L. Sima, N. Mihailescu, S. Brajnicov, A. Bonciu, A. Campean, A. Roseanu, M. Dinescu -Smart Thermoresponsive Coatings Based on pNIPAM and its Derivatives Obtained by Matrix Assisted Pulsed Evaporation, HPLA DE 2016, Promising New Laser and Optical Technologies Symposium, April 4-7, Santa Fe, USA
Oral presentations:
• V. Dinca, L.E. Sima, A. Bonciu, L. Rusen, I. Iordache(Urzica), M. Dinescu , Multiscale intelligent interfaces engineering using laser methods for mesenchymal stem cells behaviour control , EMRS 2016, Smart biointerfaces for functional biomaterials Symposium, May 2-6, Lille, France - oral presentation EMRS2016
• V. Dinca, L.E. Sima, L. Rusen, A. Bonciu, P. Hoffmann, Micro topographical surface features and gradients for influencing mesenchymal stem cells behavior for bone tissue engineering, Nanotech Washington, USA, 22-25 may-2016
• Dinca V, Rusen, A Bonciu, L.E Sima, Laser based methods for nano- and micro-engineered biomaterials for regenerative medical applications, 16 th INTERNATIONAL BALKAN WORKSHOP on APPLIED PHYSICS 7 -9 July 2016, Constanta, Romania
• L. Rusen, Livia Elena Sima, Madalina Icriverzi, N. Mihailescu, I. Anghel, A. Bonciu, S. Brajnicov, A. Cimpean, M. Dinescu, A. Roseanu and V. Dinca, In vitro behaviour of human mesenchymal stem cells on smart biointerfaces obtained by laser methods for tissue engineering applications, ICPEPA10, 28 august -2 sept 2016, Brasov, Romania
Posters:
• L. E. Sima, M. Icriverzi, K. Bohlen, E.C. Siringil, T. Jδger, K. Wasmer, P. Hoffmann, M. Dinescu, A. Roseanu, A. Cimpean, V. Dinca, Mesenchymal stem cells osteogenic fate on excimer lasers designed bone pits topographies- 10th International Conference on Photoexcited Processes and Applications - ICPEPA10, August 29 – September 2, 2016, Brasov, Romania
• M. Icriverzi, L.E. Sima, N. Dumitrescu, V. Marascu, A. Cimpean, V. Dinca, A. Roseanu, Biomimetic surface for controlled cellular response, BIOMATERIALS FOR HEALTHCARE: Biomaterials for Tissue and Genetic Engineering and the Role of Nanotechnology, 1st biennial conference BioMaH October 17-20, 2016 Rome, Italy, best poster
• M. Icriverzi, L.E. Sima, N. Dumitrescu, V. Marascu, R.W. Evans, A. Cimpean, V. Dinca, A. Roseanu, Macrophage response to biomimetic-coated material, PRIOCHEM -XII-th edition, October 27 - 28, 2016, Bucharest, Romania
• S. Brajnicov, V. Marascu, L. Rusen, A. Moldovan, V. Dinca and M. Dinescu, "Copolymer PLCL-PEG-PLCL functional bio-coating obtained by Matrix Assisted Pulsed Laser Evaporation: a deposition parametric study", 10th International Conference on Photoexcited Processes and Applications - ICPEPA10, August 29 – September 2, 2016, Brasov, Romania


Stage 3
Value: 250.370,00 lei

Objectives
Ob. 3.1 Advanced functionalized microstructured surfaces
Ob. 3.2 In vitro evaluation of the microstructured materials
Ob. 3.3 Developing the response in vitro toward implat type of surfaces

Dissemination
Invited presentations:
V. Dinca; "Bioinstructive multiscaled interfaces engineering by laser direct texturing for tailoring the mesenchymal stem cells response in vitro" Biophysics Rome 2017, 18-19 MAI 2017, Roma, Italy

Oral presentations:
1. V. Dinca, V. Mitran, S. Brajnicov, A. Bonciu, L. Rusen, M. Dinescu and A. Cimpeanu; "Graphene based composite coatings effect on MC3T3-E1 pre-osteoblasts behavior" European Materials Research Society (E-MRS), E-MRS 2017 Fall Meeting, September 18-21, Varşovia, Polonia, Session 7 - Cell-surface interactions
2. V. Dinca, L. Rusen, M. Icriverzi, V. Malheiro, L. E. Sima, M. Uta, N. Nichita-Branza, A. Roseanu, E. C. Sirigim, P. Hoffmann, M. Dinescu; "Micro-structured biointerfaces for screening mammalian cell response towards surface topography" European Materials Research Society (E-MRS), E-MRS 2017 Fall Meeting, September 18-21, Varşovia, Polonia, Session 7 - Cell-surface interactions
3. Icriverzi Madalina, Efectul materialelor compozite asupra comportamentului celular. Studii in vitro. Raport stiintific in cadrul Scolii Doctorale de Biologie, Facultatea de Biologie, Universitatea Bucuresti, 22.09.2017

Posters:
1. V. Dinca, L.E. Sima, M. Icriverzi, L. Rusen "Geometrical cues in ceramic scaffolds designed by laser texturing for human mesenchymal stem cells differentiation toward an osteogenic response" HYMA2017, 5th International Conference on Multifunctional, Hybrid and Nanomaterials; March 6-10, Lisbon, Portugal - poster P1.036.
2. V. Dinca, " In vitro cellular response of smart hybrid biomimetic coatings obtained by MAPLE " HYMA2017, 5th International Conference on Multifunctional, Hybrid and Nanomaterials; March 6-10, Lisbon, Portugal - poster P3.048.
3. L. Rusen, V. Dinca, L.E. Sima, I. Anghel, A. Bonciu, M. Dinescu and P. Hoffmann; "Spatially controlled micro patterns obtained by laser methods for cellular behavior study", EMRS2017, Section K:Cell & Tissue Nature Science, Bioengineering & Investigations; May 22-26, Strasbourg, France -poster K PI.8.
4. M. Icriverzi,L. Rusen,V. Dinca, S. Brajnicov, V.Marascu, A.Bonciu, A. Cimpean, M.Dinescu, A.Roseanu , "Tailored biomimetic nano-hibrid coatings obtained by MAPLE for inflammatory response evaluation in vitro" , EMRS2017, Section X:New frontiers in laser interaction:from hard coatings to smart materials; May 22-26, Strasbourg, France - poster X P_1.19.
5. M. Icriverzi, V. Dinca, L. Rusen, S. Brajnicov, V. Marascu, A. Bonciu, A. Cimpean, M. Dinescu, A. Roseanu, "Laser processing of hybrid biomimetic coatings",17th INTERNATIONAL CONFERENCE ON PLASMA PHYSICS AND APPLICATIONS, June 15 – 20 Magurele, ROMANIA, Topic 5: Biological and medical applications of plasmas, plasmas in liquids, poster P5-06.
6. M. Icriverzi, Livia Sima, Valentina Dinca, Anisoara Cimpean, Anca Roseanu, Hybrid Coating impact on macrophage response, 47th Annual Conference of Immunology organized by The Romanian Society for Immunology, 4-6 October 2017, Bucharest, Romania.
7. M. Icriverzi, L. Rusen,V. Dinca, S. Brajnicov, V.Marascu, A.Bonciu, A. Cimpean, M.Dinescu, A. Roseanu, Tailored biomimetic nano-hibrid coatings obtained by MAPLE for inflammatory response evaluation in vitro, EMRS2017, Section X:New frontiers in laser interaction:from hard coatings to smart materials; May 22-26, Strasbourg, France.
8. Nicu D Scarisoreanu, F. Craciun, V. Ion, A. Andrei V. Dinca, Maria Dinescu, Thin Film Obtained by Laser Techniques for Biocompatible Applications, MRS Boston 2017.